AT is usually not diagnosed until much later in life, after symptoms are manifest. Upon finding deleterious mutations in the ataxia telangiectasia mutated (ATM) gene, we confirmed the diagnosis of ataxia telangiectasia (AT) in two infants. Whole exome sequencing and analysis were performed in infants and their parents. Deep sequencing was employed to find causes of T lymphocytopenia in such infants. In addition to infants with typical SCID, screening identifies infants with T lymphocytopenia who appear healthy and in whom a SCID diagnosis cannot be confirmed. Newborn screening to identify SCID is now performed in several states. Short Abstract: Severe combined immunodeficiency (SCID) is characterized by failure of T lymphocyte development. The large number of miRNAs and complex population of small RNAs in potato suggest important roles for these non-coding RNAs in diverse physiological and metabolic pathways. The size, origin and predicted targets of conserved and potato specific miRNAs are described. Additionally, we predicted targets for all the miRNAs using the potato transcriptome data.Īs a result, 28 conserved miRNA families were found and potato-specific miRNAs were identified and validated by RNA gel blot hybridization. Conserved and potato specific miRNAs were identified and non-conserved miRNAs were validated experimentally. The candidate precursor were folded and miRNA predictions were made based on plant miRNA characteristics, such as minimum folding energy, the reads distributions of mature miRNA and star sequences, etc. The candidate precursors were excised from the potato genome at the mapping positions of these filtered reads. Small RNA reads were firstly filtered in order to remove ribosomal, transfer RNAs, snRNAs and snoRNAs. A pipeline was developed for the analysis of miRNA sequences in potato. The aim of our study is to explore the miRNA transcriptome of potato enabling to investigate the role of these small non-coding RNAs using high-throughput sequencing and bioinformatics tools to analyze miRNAs in the tuber bearing crop potato (Solanum tuberosum) from leaf and stolon tissues. Micro RNAs (miRNAs) represent a class of short, non-coding, endogenous RNAs which play important roles in post-transcriptional regulation of gene expression. Yet molecular events leading to tuberization and development are poorly understood. Short Abstract: Potato is the third largest global food crop.
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